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Assume you have a solution of mixed plant pigments; however, you are only intere

ID: 207663 • Letter: A

Question

Assume you have a solution of mixed plant pigments; however, you are only interested in the concentration of chlorophyll b. To what wavelength would you set the spectrophotometer to determine Chlorophyll B concentration. Please Explain your reasoning!

Assume you have a solution of mixed plant pigments, however, you are only interested in the concentration of chlorophyll b. To what wavelength would you set the spectrophotometer to determine the concentration of chlorophyll b? [2 pt; L2; II.C] 100 80 CHLOROPHYLL a 60 20 700 500 550 600 650 Wavelength (nm) 440 nm O460 nm 485 nm O 501 nm 640 nm 675 nm

Explanation / Answer

The correct option is 485 nm.

The absorption for chlorophyll B is maximum at 485 nm. The spectrophotometer can measure the absorption by chlorophyll B best at 485 nm, because it absorb matximum at 485 nm. The other wavelengths are least affected by chlorophyll A or carotenoids.

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