You have synthesized 3 new compounds and give them the names: Com1, Com2 and Com

Question

You have synthesized 3 new compounds and give them the names: Com1, Com2 and Com3. You conduct an Ames test and determine that all three compounds are mutagenic. You then test the capacity of mutations caused by your compounds to be reversed by other known mutagens You get the following results 5. Reversed by Mutations EMS Hydroxylamine Proflavin produced by Bromouracil No Yes Yes What conclusions can you make about the nature of the mutations produced by Comi Com2 Com3 Yes No Yes Yes No Yes No No Yes these compounds? i. Com ii. Com2 ii. Com3 You are studying the effects of mutations caused by the new compounds in question 5 You expose Drosophila larvae to either Com 1, Com2 or Com3, then collect the 6. mated them with unexposed

Explanation / Answer

Com1= Transitions

Com= Deletion of multiple base

Com3=Insertions or deletion of a single base.

Some chemical mutagen act as a base analogs for ex,5 -BU EMS and Hydroxylamine also acts as mutagen but its mode of action different from alkylating agents..its causes transitions.proflavin is a interclating agents .its causes single nucleotide -pair insertions and deletions.

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