Question 7 (0.5 points) What is the role of RNA primase? a) covalently links Oka

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Question 7 (0.5 points) What is the role of RNA primase? a) covalently links Okazaki fragments O b) alleviates supercoiling c) synthesizes a strand of RNA to use as a primer d) breaks the hydrogen bonding between strands Question 8 (0.5 points) what is the role of DNA polymerase l O Synthesizes the new strand of DNA O Synthesize an RNA primer OCovalently links the okazaki fragments O Excises RNA primer and synthesizes the replacement of DNA Question 9 (0.5 points) Why is an RNA primer necessary? O a Because DNA polymerase can only add nucleotides to the 5'end of an existing chain of nucleiotides b) Because DNA polymerase can only add nucleotides to the 3' end of an existing chain of nucleitides Because DNA polymerase can only initiate synthesis following an RNA nucleotide Because DNA polymerase is energetically unstable. Question 10 (0.5 points) What is DNA ligase function in replication and why is it necesary? Must choose more than one answer O a) Because no DNA polymerase can add nucleotides to the 5' end of an existing chain of nucleotides O b) Replaces the RNA primer with DNA c) Catalyzes the phosphodiester bond between 3. end of one okazaki fragment and 5' end of another O d) Because RNA primase is not a polymerase Question 11 (0.5 points) In eukaryotes where does replication begin? O a) Ends of the chromosomes opening to the center O b) At a single replication origin randomly placed along the chromosome. c) Simulataneously at several replication origins. O d) At the centromere working to the ends

Explanation / Answer

(7)

C.synthesizes a strand of RNA to use as a primer

(8) Excises RNA primer and synthesizes the replacement of DNA

DNA polymerase 1 removes the RNA primers from the fragments and replacing it with the required nucleotides.

(9)b.Because DNA polymerase can only add nucleotides to the 3’ end of an existing chain of nucleotides

(10)a.because no DNA polymerase can add nucleotides to the 5’ end of an existing chain of nucleotides

c)catalyzes the phosphodiester bond between 3’ end of one okazaki fragment and 5’ end of the other.

DNA ligase seals the break in the phosphodiester bond, joining the newly synthesized Okazaki fragment,

(11)

C.simultaneously at several replication origins

DNA replication is initiated from specific sequences called origins of replication, and eukaryotic cells have multiple replication origins

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