derson 1 6. The primary function of standard calibration is: a. To standardize t

Question

derson 1 6. The primary function of standard calibration is: a. To standardize the migration pattern of a protein on the gei b. To standardize the response of the instrument c. To standardize the extraction step of proteins d. All of the above Dr Sele that To If the amount of the protein TNF-a measured is 100 pg, it is equivalent to: a. 100 x 1o ng b. 100 x 109 mg c, 100 x 106 g d. All of the above 8. If the absorbance of a cellular supernatant sample is 0.6, using the standard calibration equation (best-fit line eq,) Y 0.004 X + 0.01, the calculated concentraticor (expressed in pg/ml) of the target protein is: a. 265 pg/ml b. 62.5 pg/ml c. 225 pg/ml d. All of the above 9. ELISA uses the following antibodies: a. Biotinylated antibodies b. Substrate c. Polymers d. None of the above 10. Good and safe laboratory practice include: a. Wearing gloves b. Eating and drinking in the work area c. Wearing goggles when needed d. a and c only 11. The Primary BSA Standard concentration is 4 mg/ml, how much is the volume aliquot needed to prepare a 20L working BSA of 1 gfuL: a, 10 c.1 d, 20 12. In plate ELISA, streptavidin is used to: a. Bind the target protein to the antibody b. Bind the Horse Reddish Peroxidase to the target protein antibody c. Bind the target protein to the Horse reddish peroxidase All of the above

Explanation / Answer

The primary fuction of standard calibration is to standardize the migration patter of proteins in the gel.

This is calculated through a graph x axis and y axis - based on the movement of proteins in the gel they are standardized.

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