·V- 97. The simple staining procedure can be successfally carried out with which
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·V- 97. The simple staining procedure can be successfally carried out with which of the following dyes? Methylene blue B. Nigrostn C. Eosin Crystal violet Either methylene blue or crystal violet 38. Many media in microbiology are called defined media because A the exact composition of the individual amino acids, vitamins, growth factors other components are not exactly known other components are exactly known C. they contain special constituents to stimulate the growth of fastidious microorganisns D. they contain special constituents to support anaerobic growth 39. A disinfectant should be used on the laboratory work surface A before the beginning of laboratory work B. after all work is completed C. after any spill of live micro-organisms D. should apply to all of the above E should apply only to A and B Bacteria can be classified into 5 groups based on their requirements for air that contains 20% of oxygen. Which of the following are examples of facultative anaerobes? 40, A. Bacillus sp. and Pseudomonas sp. B. Staphylococcus sp. and Escherichia coli C. Streplococcus sp. and Helicobacter pylori D. Clostridium sp. and Bacteroides sp 41. A strip of was introduced into a GasPak anaerobic jar to serve as a visual oxygen indicator during incubation. The strip will turn if oxygen is present in the jar. Methyl red; red C. Methylene bruc, biue D. Malachite green; green Methylene blue; colorless Nambers 42-46 Match the colors below to the expected experimental results: A. Pink B. Red C. Blue D. Green E Purple f Staphylococcus aureus after successful Gram stain experiment PufrLe negaerium after succesuful Grem stain experiment PuRpue Color of Bacilus after 4 Coler of Bcherichia coli after successful acid fast stain experimentExplanation / Answer
Considering that answers for question 40 and 41 are not marked, answers for the same are being provided.
40) The correct answer should be (B) as both Staphylococcus species and E.coli are examples of facultative anaerobes.
Some other examples for facultative anaerobes are Streptococcus species, Brewer's yeast, Corynybacterium.
41) Gas Pak are used to culture the anaerobic bacteria.
To ensure that no oxygen is present in the jar, a strip of methylene blue in inserted which turns blue if oxygen is present (due to oxidation) and turns colorless, if no oxygen is present (indicating anaerobic environment has been achieved).
So, the correct answer should be (C).
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