rential staining procedute 7 Which of the following options most options most ac

Question

rential staining procedute 7 Which of the following options most options most accurately represent n diffarenti A Gram stain B. Acid-fast stain C. Schaeffer-Fulton spore stain Outy Gram stain and Acid fast stain All are differential staining procedures. op sach au methylene blue bind to cellular molecules that are A hydrophobic 8. Basie dyes s negatively charged positively charged. D, magnetic 9·The Dormer procedure is used to stain A lagella. fat deposits. endospores. D. DNA of chromosomes. 10. Small internal cell structures are best visualized with a A. light microscope. dark-field microscope. transmission electron microscope. flagellar microscope. 11. The two sets of magnifying lenses found in a light microscope are the A basic and transverse. condenser and occular. ocular and objective. D. simple and phase. 12. In viewing a microscopic specimen under oil immersion objective, oil is used to A. increase the refraction. C. increase the reflection. D increase the resolution. E decrease the refraction AND increase the resolution. 13The order of reagents in the Gram stain reaction is A. safranin, alcohol, methylene blue, iodine. B crystal violet, iodine, alcohol, safranin. ylene blue, alcohol, safranin. crystal violet, alcohol, iodine, safranin. 4 The cell wall of Gram-negative organisms has a thick peptidoglycan layer,-- as a thin peptidoglycan layer C is more permeable to various molecules than the Gram-positive cell wall. E, has a thin peptidoglycan Tayer AND is characterized by an outer membrane containing LPS. D. is characterized by an outer membrane containing LPS.

Explanation / Answer

And 7. All are example of differential staining.

Ans 8. Basic dyes which are positively charged binds to negatively charged molecules.

Ans 9. Domer staining is used to stain endospores.

Ans 10. Small internal structures are visualized with transmission electron microscope.

Ans 11. Ocular and objective lens

Ans 12. Increase the resolution. Oil of high refractive index is used to increase the numerical aperture of objective lens in order to visualize the object with better resolution.

Ans 13. First cells are stained with Crystal violet followed by iodine that binds with Crystal violet and stains the cell. This is followed by alcohol to remove excess stain. Last saffarnin the counter stain is added.

Ans 14. Cell wall of Gram negative bacteria has a thin peptidoglycan layer and outer LPS leaflet.

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