1. (4pts) In this picture of an SDS PAGE (see below): Question Answer a. Ladder
ID: 207206 • Letter: 1
Question
1. (4pts) In this picture of an SDS PAGE (see below): Question Answer a. Ladder and antibody samples have been loaded into different b. The range (smallest to largest size) of the ladder is: e. The approximate size (in kDa) of "Heavy Chain" is: d. The approximate size (in kDa) of·Light Chain., is: on the gel. SDS PAGE of Antibody Proteins ("Heavy Chain" and "Light Chain") with Ladder kDa -heavy chain -light chain 38 28- 2(2pts) This gel has approximately 10 of protein in each lane. What would this gel look like if you loaded lug of protein in each lane? What would this gel look like if you loaded 100ug of protein in each lane? a. b. 3. (2pts) This gel was run for 90 minutes. What would this gel look like if we only ran it for 30 minutes? What would this gel look like if we ran it for 3 hours? c. d. 4. (2pts) How is the protein ladder similar in function to the Protein Standards that we used in the Bradford Assay?Explanation / Answer
1.
a. Ladder and protein samples are loaded in different LANES.
b. Range of the ladder = 3 kDa to 188 kDa
c. Approximate size of heavy chain = 25 kDa
d. Approximate size of light chain = 50 kDa
2.
a. If 1 ug of protein was loaded, the band intensity would be 10 times less.
b. If 100 ug of protein was loaded, the band intensity would be 10 times more.
3.
a. If the gel was run only for 30 min, the band would run only for a short distance and may not be properly resolved.
b. If the gel was run for 3 hours, the bands would run longer.
4. The ladder is used to compare the sizes of unknown bands.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.