1.Our tyrosinase is from a potato and our primary antibody was generated in a sh
ID: 207142 • Letter: 1
Question
1.Our tyrosinase is from a potato and our primary antibody was generated in a sheep, therefore our secondary antibody should be derived from potato to be specific for the potato.
True or False
2. The primary tyrosinase antibody we are using is polyclonal. This means each antibody recognizes the same epitope on tyrosinase.
True or False
3. A student spends all day performing a Western blot. After incubation with the substrate, the student visualizes no protein (and is not very happy!). Which of the following scenarios is likely NOT the cause of the problem?
a. The student did not effectively transfer the proteins from the gel to the membrane.Explanation / Answer
1. False
Secondary antibody should be raised in a species other than the species of the primary antibody.
2. False
Polyclonal antibodies recognize multiple epitopes.
3. Option C is correct.
No membrane transfer, Not enough sample and non-specific primary antibody = No band in the gel.
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