12 A) Which of the following is the correct order of events in a Western blot? S

Question

12 A) Which of the following is the correct order of events in a Western blot?

SDS-PAGE > membrane transfer > detection > antibody incubation

antibody incubation > SDS-PAGE > membrane transfer > detection

SDS-PAGE > membrane transfer > antibody incubation > detection

SDS-PAGE > antibody incubation > membrane transfer > detection

12 B) Which of the following are key features of a mass spectrometer?

1. mass analyzer

2. ion source

3. detector

4. spectrophotometer

5. computer

A) 1, 2, 3, and 5

B) 1, 2, 4, and 5

C) 1, 2, 3, and 4

D) 1, 2, 3, 4, and 5

Explanation / Answer

12.

The correct order of the western blot is:

First perform SDS page, then transfer the membrane, then incubate with antibody and then detect using uV machine.

Option c is the answer

The key features of mass spectrometer include:

Ion source, computer, detector and a spectrophotometer.

Option d

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