What can conclusions can be made? How does pH affect this experiment? What would

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What can conclusions can be made? How does pH affect this experiment? What would be the PI here? What amino acids would likely react where the problem states there is a change in the picture? Are there any purification techniques that can be done to separate the protein of interest?

pH pH I perform a 2D gel electrophoresis of proteins from the eggs of two different species of birds. I then immunoprecipitate the protein marked "X" in species 1 using an antibody specific to that protein. The same antibody immunoprecipitates the protein marked "Y" in species 2. After treating the purified proteins with Glycosidase, they run identically on the 2D gel. Sequencing shows that X and Y have identical amino acid sequences. 13 13 300 ! 10 E 10 Species 1 Species 2

Explanation / Answer

Where is the picture..

There is no pictures given in question plz provide picture so that i can answer ur questions.

Thank u

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