6. Jane was very careful in performing her Petri dish inoculation. When she exam

Question

6. Jane was very careful in performing her Petri dish inoculation. When she examined her plate during the next lab period, a fuzzy white colony was growing on her plate, but not along her inoculation streak What happened? Where did this colony come from? 7. Jeffrey, Jane's partner, inoculated a tryptone broth tube with E. coll. When he examined his broth tube during the next lab period, there was no growth at all. What could have gone wrong with his experiment? 8. In the case file, the organism responsible for the patient's death is a normal member of the human microbiota How do you explain it causing the death of this patient? t is the connection of the events described in the case file and today's laboratory activity on aseptic technique 9. Wha

Explanation / Answer

6. Maybe Jane left it open, so fungus from air grown up in the inoculating petri dish. Or she did not sterilize properly her inoculating instrument and petri dish between streaking and contaminated her culture.

7. He probably did not allow his loop to cool adequately. A very hot loop can kill your inoculum.

or He could have not actually removed any inoculum (missed dipping his loop into the broth).

or The culture could possibly have been "dead".

8.

We know that in our intestine E.coli is present and it is harmful to our intestine still it resides in our intestine. But the main problem arises if it's number increases way too large, that causes infection and this can even lead to death. So, even the normal microbiota needs to be in control quantity in our body.

9. It is important because it helps eliminate contaminants that could cause unreliable results, it protects us from contaminating our environment, ourselves, and others. In the case file and the laboratory activity, aseptic techniques are used to prevent contamination. Bacteria are ubiquitous.

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