8. Filter the homogenate through four layer thickness cheesecloth in a clean 250

Question

8. Filter the homogenate through four layer thickness cheesecloth in a clean 250 ml beaker. Keep filtrate on ice. Where do you think most of the onion DNA is present? Filtrate or material retained on cheesecloth containing DNA interface Step II. De-proteinization 9. Transfer 50 ml of filtrate into a clean 250 ml conical flask. 10. Add 10 ml chloroform into the conical flask. You should see a layer of chloroform separated from the homogenate at the bottom of the flask. 11. Gently swirl the content of the flask a few times. DO NOT VIGOROUSLY MIX SOLUTION What happens at this stage?

Explanation / Answer

In filtrate, most of the onion DNA is present.

At that stage, we will see a white precipitate appears at the interface of the two solutions (interface between chloroform and aqueous homogenate containing DNA). This precipitate is the denatured protein.

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