Procedure: Starch Agar -Starch Hydrolysis (5pts) Obtain a starch agar plate and

Question

Procedure: Starch Agar -Starch Hydrolysis (5pts) Obtain a starch agar plate and label the bottom in the following manner (remember initials and section number): 1. 2. Using an inoculation loop, you will inoculate each labeled area as shown above. 3. In 24 48 hours, check the plate for growth. Flood the plate with Gram's iodine. Areas of starch hydrolysis will appear clear while un-hydrolyzed starch will remain blue-black. Record your results: 4. Starch Hydrolysis Color of Medium Organism Growth Starch Hydrolysis (+or- ) Bacillus subtilis Pseudomonas aeruginosa Escherica coli

Explanation / Answer

If the agar with unhydrolyzed starch is flooded with the Grams iodine, the iodine molecule will slip inside of the amylose coil present in the starch. This turns the color of the agar into blue-black color.

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