1-The polymerase chain reaction (PCR) is used A. to produce large numbers of ide
ID: 205575 • Letter: 1
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1-The polymerase chain reaction (PCR) is used A. to produce large numbers of identical DNA segments called gene probes. B. increase the amount of DNA to be searched in DNA sequencing procedures C.in water-quality tests D. in HIV and HPV detection E. to identify even poorly growing bacterial cultures F. in all of the above 2- Which of the statements below are false with respect to gene probe technology? A. Since first introduced in the 1970s, gene probe tests have been met only with periods of unbridled enthusiasm. B.The ViraPap test is a commercially available gene probe test for detecting human papilloma virus (HPV), the virus that causes genital warts. C. A similar technique can be used to conduct water-quality tests based on the detection of coliform bacteria such as E. coli D. gene probes and PCR have been useful is in the detection of human immunodeficiency virus (HIV) E. None of the above 3- When gene probe analysis becomes more widely accepted it will allow the identification of A. Salmonella, B. Shigella C. Vibrio D. all of the above 4- Gene probe technology A is described as "exquisitely accurate" B. have a high degree of discrimination and reliability as strong as older identification methods. C allows one to get a result in a few short hours instead of many days D. is extremely sensitive E. all of the above 5- Gene probe assays are widely available in kit forms for a variety of bacterial species except A. Staphylococci B. Haemophilus C Listeria D Mycobacterium, and Neisseria E fungi (such as Blastomyces, Coccidioides, and Histoplasma). 6. The future value of gene probes will depend in part on A. the development of ways to minimize false-positive reactions due to contamination B. methods of increasing the sensitivity of tests C. on mechanisms for enhancing the signals from probes bound to their target molecules. all of the above E A & C onlyExplanation / Answer
Polimerase Chain Reaction (PCR) is a process used in genetic labs to replicate copies of a strand of DNA using the Taq Polimerase enzyme from Thermus aquaticus, a termophilic bacteria.
This is exactly the process that takes place in organisms to replicate DNA and it only stops when source on the reaction are off, or the reaction is stopped by cooling it. Large amounts of DNA are generated by PCR. This is very useful when low amounts of DNA are available, and the protocol can be used to increment the amount of DNA for sequencing procedures.
By using PCR, is possible to detect organisms even when their populations are small by increasing the amount of their DNA, for example, bacteria in water samples or in growing cultures. This mean that the PCR protocol can be used in water quality tests as well.
Human Immunodeficiency Virus (HIV) has not its own DNA because viruses only synthesizes RNA, but when humans are infected with HIV, this RNA is converted into DNA by the Virus’s enzymes, so it can also be replicated and detected by the PCR process. HIV detection is commonly implemented by the PCR process.
That’s why the correct answer for question 1 is F.
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