you have identified a gene that when mutated, causes stunted growth in mice. You

Question

you have identified a gene that when mutated, causes stunted growth in mice. You have cloned the wild-type mouse gene into bacteria. You have wild-type

mice and mice with the mutated gene, and you have the tools for making transgenic and knockout mice. You hypothesize that when the gene product is present in above normal amounts, the mice would grow abnormally large. You set up an experiment to test this hypothesis.

a. Would you start with wild-type mice or mutated mice? Why?

b. Would you make transgenic mice or knockout mice? Why?

Explanation / Answer

To check whether ectopic expression/overexpression of the given gene causes abnormally large body, we should use mutated mice. This is because of the fact that the mutant mouse lacks the endogenous gene. So, there will not be any interference from it.

To overexpress a gene, we should generate transgenic mice harboring the construct.

Knock out strategy is used to produce deletion mutants.

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