Laboratory 6: Analyses of Succinate Dehydrogenase Activity Data: Statistics and
ID: 205430 • Letter: L
Question
Laboratory 6: Analyses of Succinate Dehydrogenase Activity Data: Statistics and Hypothesis Testing Pre-lab questions (based on data from last week's lab) It is possible that, in the reactions without succinate (reaction 1) of the SDH assay, the absorbance could increase after the 10-minute incubation. Explain what would be occurring in the homogenate to allow that to happen. 1. 2. What does the reaction with no malonate (reaction 2) show? There are two different statistical analyses that need to be done with the data from reactions containing malonate. Describe each, making clear how they give different information. 3.Explanation / Answer
Answer:
1) Explain what would be occurring in homogenate to allow that to happen?
2) What does the reaction with no Malonate show?
Malonate is a competitive inhibitor of Succinate dehydrogenase enzyme. Reaction that doesn't contain any Malonate will be un-inhibited and the rate of reaction will be maximum in this case due to the absence of inhibitor. This value can be used in the assay as the reference value to determine the extent of reduction in activity (and rate of reaction) using different concentrations of Malonate (dose response curve).
3) There are two different statistical analysis that needs to be done with the data from reactions containing malonate. Describe each, making clear how they give different information?
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