1. Application question: Why is succinate important when explaining that E. coli

Question

1. Application question: Why is succinate important when explaining that E. coli evolved to take up citrate under

conditions with oxygen?

2.Fully describe the E. coli cit operon as it functions in conditions without oxygen. Include promoter, repressor and

operator in your description.

3. How did this new arrangement facilitate the transcription of the citT primary transcript under conditions without oxygen?

How did this new arrangement facilitate the transcription of the citT primary transcript under conditions with oxygen?

4. Synthesis question: Explain the advantages and disadvantages of the citrate operon mutation in E. coli living in a flask with E. coli that do not have this mutation.

Explanation / Answer

1. Mutation in the citT gene that is responsible for coding citrate –succinate antiporter that is responsible for E.coli evolution in aerobic condition. Hence, in this way succinate important when explaining that E. coli evolved to take up citrate under conditions with oxygen.

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