general biology manual 2nd B. pH and enzyme activity In this part of the exercis
ID: 204695 • Letter: G
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general biology manual 2nd B. pH and enzyme activity In this part of the exercise, we are going to examine what effect pH has on the rate of this enzyme-cataly red reaction. To do this, we will perform the reaction in different ph environments Experimental Setup. 1. Label four test tubes and mark them each with a number, 1-4 3. Next, add the following solutions to the proper tube and gently stir. 2. Add 10 drops of liver solution to each tube. #1 #2 #3 #4 10 drops ofpHl solution 10drops of pH 6 solution 10drops of pH 8 solution 10drops ofpH 14 solution What is the independent variable in this experiment? Why? What is the dependent variable in this experiment? Why? Which factors have been controlled/standardized among the tubes? Which test tube serves as the control? Why? What do you predict will happen to the enzyme activity in each test tube and why? Test tube #1 Test tube #2 Test tube #3 Test tube #4 4. Add 0.5 ml of H O; to each tube and record the results in Data Table 2 TABLE 2: Reactivity (height of bubble column in inches) pH I pH 6 pH 8 pH 14 Was your prediction supported by your data? ExplainExplanation / Answer
Q1) CELL THEORY
In 1838, Matthias Schleiden, a German botanist examined a large number of plantsand observed that all plants are composed of different kinds of cells which form the tissues of a plants. At about the same time, Theodore Schwann in 1839, a British zoologist studied different types of animals cells and reported that cells had a thin outer layerwhich is today known as the 'plasma membrane.' He also concluded, based on hids studies on plant tissues, that the presence of cell wall is a unique character of the plant cell. On the basis of this, Schwan proposed the hypothesis that the bodies of animals and plant cells are composed of cells and the products of cells. Schleiden and Schwan together formulated the cell theory. This theory however, did not explainas to how new cells were formed. Rudolf Virchow in 1855 frist explainesd the cells divided and new cells are formed from pre - existing cells. He modified the hypothesis of Schleiden and Schwan to give the cell theory a final shape. Cell theory as understood today is:
a) all living organisms are composed of cells and products of cells
b) all cells arise from pre - exixting cells.
Q2) prokaryotic cells eukaryotic cells
- A prokaryotic cell is a single membrane system. -Eukaryotic cell is a double membrane system.
- Ribosomes are 70s lie free in cytoplasm. - Ribosomes are 80s lie free or bound to ER.
- Cell membrane bears respiratory enzymes. - Cell memebrane lacks respiratory enzymes.
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