How can you ensure that a bacterium did not take up an empty vector during the c

Question

How can you ensure that a bacterium did not take up an empty vector during the cloning process?

Insert your target gene in the middle of the lacZgene and use a color indicator to make sure that lactose fermentation is not possible.

Insert your target gene in the middle of the lacZgene and use a color indicator to make sure that lactose fermentation is still possible.

Insert your target gene outside of the lacZ gene and use a color indicator to make sure that lactose fermentation is not possible.

Insert your target gene outside of the lacZ gene and use a color indicator to make sure that lactose fermentation is still possible.

Explanation / Answer

Inserting the target gene in the middle of the lacZ gene MCS location then growing it to see if blue or white colony appears is the method. So option B is correct.

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