1. You are a member of a laboratory studying the development of the vertebrate n
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1. You are a member of a laboratory studying the development of the vertebrate nervous system. You are specifically interested in the molecular mechanisms underlying the separation of the neural tube from the overlying epidermal ectoderm during neural morphogenesis. D Folling MHP Eevation apoove DEVELOPMENTAL OLO,Figure 94 Pat To investigate this process, you isolate neural and epidermal ectoderm, and perform microarray analysis to identify mRNAs that are expressed in the neural ectoderm, but in the epidermal ectoderm. One of the mRNAs you identify in this experiment encodes a protein related to epithelial cadherins, which you cleverly name N-cad (Neural-cadherin) a. Propose a hypothesis to explain the possible role of N-cad in the development of the vertebrate neural tube.Explanation / Answer
a.Hypothesis loss of the function of N-catherine disrupt the formation of neural tube.
In vertebrates if the level of N-catherine is too low or the protein is less expressed, the neural tube is not properly formed. Hence the development of brain and spinal cord is affected by low level of N-cad.
b. Experimental set up.
Design know out mice which have lack in N-cad gene and Linc7c gene (N-cad/Linc7c, -/-) cross this mice with the normal mice having normal N-cad and Linc7c gene +/+). The progeny having lack of N-cad and linc7c gene may have developed defective neural tube and normal mice develops the normal form of neural tube. Which indicates that N-cad and Linc7c gene are important for development of neural tube.
c. In vertebrates loss of the function of N-cad results in abnormal neural tube formation.
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