4. You perform a PCR reaction using the following template DNA and primers. What

Question

4. You perform a PCR reaction using the following template DNA and primers. What size(s) of products do you expect? Explain vour answer. Primer: 5'-ctctgggctc agagcagccc-3 Primer: 5'-catgcagccg tcctgatctc-3 Template: 1 attatatttt atattatata ctctgggctc agagcagccc 40 41 atattatata tatatatttt aaaatattat aaatttattt 80 81 catgcagccg tcctgatctc attatatttt ataatttttt 120 121 ttttattttt attatatttt ttttttattt ttattttttt 160 161 aaaatattat tatattatta taattaattt attataaaat 200 201 tatatatttt attatatttt tatatatttt attaaatttt 240 241 attatatttt tttttaattt ttattattat tttttatata 280 281 tatatatttt tatatatttt attatatttt attatatttt 320 321 tttatataaa attatatttt tatttttttt ttttttataa 360 361 gggctgctct gagcccagag attatatttt attataaaat 400 401 attatatttt attataaaat aaaatataat aaaatattat 440 441 aaaatattat aaaaaaaaat gagatcagga cggctgcatg 480 481 atattatata atattatata attatatttt atattatata 500

Explanation / Answer

Ans Product size depends on the sequence from which designed primers. If, sequence of 500 bp of DNA Template and designed primer from starting and ending sequence of that DNA then the PCR product size would be about 500bp.

Here, template is 500 bp. forwaring primer start amplify from 21bp to 480bp (reverse primer start at 480bp) . So amplified product size will be 460bp.

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.