Sex determination in Mammals. You have identified a gene that you have named \"m

Question

Sex determination in Mammals. You have identified a gene that you have named "mont1" (for ‘make ovaries not testes') that is expressed at the time when the bipotential gonad becomes specified to become an ovary. Because of this "show it" evidence that mont1 is expressed in the right place at the right time, you hypothesize that mont 1 is a critical regulator of the female primary sex determination pathway to specify ovary development. c. From what you know about the primary sex determination pathway for ovary development, what signaling pathway would you predict may activate mont1 expression? d. What "lose it" experiment could you do to determine if mont1 is necessary for ovary development. Briefly describe the expected result. (As in I would do X experiment and expect to see Y result if mont1 is necessary for ovary development) e. What "move i" experiment could you do to determine if mont1 is sufficient for ovary development. Briefly describe the expected result. (As in I would do X experiment and expect to see Y result if mont1 is sufficient for ovary development)

Explanation / Answer

c)

The Wnt signaling pathway is the main pathways responsible for determining ovary development. ‘mont1’ gene expression will be activated via this pathway. First Wnt protein activates Dvl (Dishevelled) protein; this through a series of steps activates - catenin. This protein moves into the nucleus and activates ‘mont1’ gene; this further starts ovary development.

d)

‘mont1’ is most probably activated by - catenin. If you disallow ‘- catenin’ protein to attach to the promoter, then it will not be able to synthesize the ‘mont1’ mRNA which is necessary for ovary development.

So, if you perform ‘-catenin’ ‘lose it’ experiment, then, ovary development will not occur.

e)

If you perform ‘- catenin’ ‘move it’ experiment, then, ovary development will occur.

This will finally prove that -catenin and ‘mont1’ is necessary for ovary development.

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