1. Under what condition that a bacterial cell with pGLO plasmid will glow under
ID: 204385 • Letter: 1
Question
1. Under what condition that a bacterial cell with pGLO plasmid will glow under UV light?
a. presence of Ca+ ions
b. presence of ampicillin
c. presence of arabinose
d. after the heat shock treatment
2. A bacterium successfully transformed by pGLO plasmid will be able to grow on ampicillin media.
a. true
b. false
3. In nature, transformation is completed when:
a. bacteria uptake a foreign DNA
b. foreign DNA molecules enters bacteria and recombine with bacterial genome
c. foreign DNA enters bacteria through bacteriophage
d. bacterium receives DNA fragment from sex pilus of donor bacterium
4. if 1ug of plasmid DNA is able to transform 200 bacterial colonies, what is the transformation efficiency?
a. 50
b. 100
c. 200
d. 0.005
5. What is the function of bla gene?
a. breakdown the ampicillin
b. regulate the transcription of GFP gene
c. express the fluorescent trait in bacteria
d. control the uptake of DNA molecules in bacteria
6. We can transform a bacterium by allowing it to uptake fragments of target DNA from the media, or transform it with plasmids that carry the target DNA fragment. Which of these method is more efficient and has higher success rate?
a. transform with plasmid
b. uptake fragments of target DNA from the media
c. both methods are equally efficient
d. cannot be determined
7. The process of bacterial cells uptake DNA chromosome fro environment is called transformation.
a. true
b. false
8. The pGLO plasmid consists of which of the following genes?
a. blar gene
b. GFP gene
c. araC gene
d. none of the above
9, Bacterium that contains a pGLO plasmid will be able to produce ampicillin resistance protein, ?-lactamase.
a. true
b. false
10. How do we make the bacterial cells more competent to receive foreign DNA? Select the best treatment from the options below:
a. treatment with positively charged calcium chloride (CaCl2)
b. lower the pH
c. heat shock
d. treatment with calcium ions and heat shock
Explanation / Answer
1.Correct answer is :c. presence of arabinose
2.Correct answer is:a. true
3.Correct answer is:a. bacteria uptake a foreign DNA
4.Correct answer is:c. 200
5.Correct answer is:a. breakdown the ampicillin
6.Correct answer is:a. transform with plasmid
7.Correct answer is:a.true
8.Correct answer is:b. GFP gene
9.Correct answer is:a.true
10.Correct answer is:d. treatment with calcium ions and heat shock
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