B. B C. A or B D. A and B E. It depends if the organism is prokaryotic or eukary

Question

B. B C. A or B D. A and B E. It depends if the organism is prokaryotic or eukaryotic. 7. You have performed the following mating experiment using Hfr and F- strains of Escherichia coli: Hfr (thr+ leu-strs) × F-(thr-leu-str), which of the following selective media would you use to grow only recombinant colonies? A) Complete media B) Minimal Media C) Minimal Media + streptomycin D) Minimal Media + leucine E) Minimal Media + streptomycin + leucine 8. All of the following enzymes are involved in DNA replication. Of those listed, which is third in the order? A) DNA helicase 2

Explanation / Answer

The F- strain does not have fertility factor. The streptomycin medium will be used for screening hfr variants that have been able to survive. Since, the F- factor will render the hfr not able to grow in leucine or threonine. Hence, the answer will be:

C) minimal media + streptomycin

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