uestion 1 Fill in the blanks (15 pointsy 2. The temperature at which half the DN
ID: 203980 • Letter: U
Question
uestion 1 Fill in the blanks (15 pointsy 2. The temperature at which half the DNA helical character is loss i. retried- 3. The procedure of DNA renaturation after melting is called s. 4. DNA polymerase reads the template strand in the as palindromic sequences cleave DNA at sites with inverted repent sequenes ve 6. A protein is considered to be structure without its normal activity. when it is converied into a randomlby coiled e amino acidsAsp and Lys are being separated on a Positively Charged on eel te with a buffer with a pH of 7.0. Which amino acids (The PI of Asp- 2.77; Lys-9.74) A pyrimidine found in all DNA but in only some RNA is exchange colmn 8. 24. The mole fraction of For the same strand [T]+I(C] will equal to 50% of DNA is 9. one of the DNA strand of a double-helical DNA is IA-03 and 101 10. A solution of DNA is being heated slowly until the melting temperature is reached. At this poin 11. Spontaneous deamination of cytosine will result in formation of 12. When DNA is heated to 95 degree Celsius, N-glycosidic bonds will 13. Pseudouridine is widely found in RNA. TRUEor FALSE 14. UV light can induce 15. PCR can be used to generte point mutants. TRUE or F break. or FALSE TRUE of pyrimidine or FALSEExplanation / Answer
Answer:
2) The temperature at which half the DNA helical character is lost is referred to as Melting temperature Tm
3) The procedure of DNA renaturation after melting is called Annealing.
4) DNA polymerase reads the template strand in the 3' -- > 5' direction
5) Restriction endonucleases cleaves DNA at sites with inverted repeat sequences referred to as palindromic sequences
6) A protein is considered to be denatured when it is converted into randomly coiled structure without its normal activity.
7) The amino acids Asp and Lys are being separated on a positively charged ion exchange column with a buffer with a pH of 7.0. Which amino acids Lysine will elute first?
8) A pyrimidine found in all DNA but in only some RNA is thymidine
9) The mole fraction of one of the DNA strand of a double-helical DNA is [A] = 0.3 and [G] = 0.24. For the same strand [T]+ [C] will equal to 0.46.
10) A solution of DNA is being heated slowly until the melting temperature is reached. At this point 50% of DNA is single-stranded and 50% is double-helical
11) Spontaneous deamination of cytosine will result in formation of Uracil
12) When DNA is heated to 95 degree Celsius, N-glycosidic bonds will hydrolyse.
13) Pseudouridine is widely found in RNA. TRUE
14) UV light can induce dimerization of pyrimidine
14) PCR can be used to generte point mutants. TRUE
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.