(2) You need to 2.5 Lof60% EOH but all you have available is 90% EtOH and 100L o
ID: 203958 • Letter: #
Question
(2) You need to 2.5 Lof60% EOH but all you have available is 90% EtOH and 100L of DI water. How much of each compound should you use to make your desired solution. 50 ml of 0.25M NaOH solution. How much (3) You have a bottle of NaOH (solid; MW-40) and need to make 1 NaOH should you use? (4) Using the mate rials below, calculate and write instructions on how to make a 100 ml solution containing 2.5M chloroform, 18% dimethylene chloride, and 10 mM cytoplasmic stain. Since this is a non-polar solution, assu ether will be your solvent. A bottle of 4M chloroform (liquid) A bottle of 90% dimethylene chloride (liquid) A container of 10 ml of 1M cytoplasmic stain (liquid) Unlimited supply of ether - -Explanation / Answer
Answer:
(2)
Stock concentration= 90%
Required concentration= 60%
Required Volume= 2.5L
C1V1=C2V2
(Stock) (required)
V1= C2V2/C1
V1= (2.5L x 60%)/90% = 1.66L
Take 1.66L of 90% stock solution and add 840 mL water to get 60% solution,
(3)
Grams=Molarity x (Volume x Molecular weight)
=0.25 M x 0.15 L x 40
=1.5g
1.5 g of NaOH dissolved in a total of 150 mL gives 0.25 M solution.
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