ProccOD Typically, we run samples in tp non-template control, for each mastermix

Question

ProccOD Typically, we run samples in tp non-template control, for each mastermix. While PCR UL quantification down into the pico-gram range, typical assays are run with 40- 120 ng of RNA per reaction. Adjust RNA concentrations 1. Decide on an appropriate RNA amount per reaction (M) and decide on the volume of RNA d: (Vrn) per reaction. Typical values are 40-120 ng per reaction and 1-3.9 L per reaction. Determine your concentration : [RNA] = M/Vrxn 2. 3. " Determine how much RNA solution you need: Vtot Gol 3+extra, where Gol is the number of genes you will be amplifying, 3 is to ensure you have enough for triplicates, and extra is just several microliters to ensure you don't run out. 4. Determine how much of your RNA samples (Vsamp) and how much DEPC treated water (Vh2o) you need to add to make up Vtot for each well. Vsamp Vtot [RNA/IRNA sample] where IRNA sample] was quantified previously. Vh20 Vtot-Vsamp. Make up labeled tubes and ad the appropriate Vsamp and Vh20 to each tube, one for each 5 sample.

Explanation / Answer

Depending on the data 40-120ng of RNA you will required per reaction which is the concentration of your RNA in the total reaction mixture and for achieving that concentration (40-120ng) you have to add 1-3.9micoliter RNA solution per reaction, which is the final volume.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.