atory Report 35 Miclecular and Chromoscmal Genetics to locate specific gene loci
ID: 203576 • Letter: A
Question
atory Report 35 Miclecular and Chromoscmal Genetics to locate specific gene loci on DNA segments. Use the codon e. Circle the located gene, and indicate the probe that was successful in ecular biologists use probes of mRNA ences determinesd in 3c as probes to loca mplate (single-strand) DNA sho locating it. gene (complementary base sequence) in the segment o A-G THT-C TIT-A CiC.CTHG A-AC G GICA-TT.CA G-A-CtA-T-TIC-CGCT-A G ACHCA TIT-A-CHT T-CH Successful probe nsider the following hypothetical gene. Indicate the imRNA codons that it forms and the sequence of amino acids produced in the polypeptide chain. T-A-CITT-AGA-AA T-A-C.C-G:A-A-GA-CT mRNA codons Amino acid sequence the following mutations, show the effect by indicating the m polypeptide 1. Base substitution For RNA codons and the amino acid sequence in the T-A-CIT-T-AtG-A-GtA-T-AC-C-GtA-A-GHA-C-T mRNA codons Amino acid sequence 2. Triplet addition -T-A-Ct T-T-A-G-G-T+ G-A-AA-TAC-C-GA-A-GA-C-T- mRNA codons Amino acid sequence 3. Base addition mRNA codons Amino acid sequence 4. Base deletion (site indicated by arrow -T-A-C+T-T-A+G-A-AHA-T+C-C-GHA-A-GHA-C.T mRNA codonsExplanation / Answer
AS PER CHEGG POLICY, PROCEEDING TO ANSWER ONLY THE FIRST 4 MARKED QUESTIONS/SUBPARTS, THAT IS, QUESTIONS e TO f.2:
Original Sequence: TACTTAGAAATACCGAAGACT
ANSWER e
mRNA Codons: UAC UUA GAA AUA CCG AAG ACU
Amino Acid sequence: Y L E I P K T
ANSWER f.1 Base Substitution
mRNA Codons: UAC UUA GAG AUA CCG AAG ACU
Amino Acid sequence: Y L E I P K T
The effect is a silent mutation as both the original and the affected codon code for the same amino-acid.
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