Proflavin (PRO) is an intercalating agent that can cause single base additions o

Question

Proflavin (PRO) is an intercalating agent that can cause single base additions or deletions. 5-bromouraail U) is a base analog that causes both CG-to-TA and TA Frank, a sloppy scientist, carried out a series of E. colimutagenesis experiments to study the specificity of Unfortunately, he forgot to label the plaie from which each mutant originated G transitions. Hydroxylamine (HA) is a agent that causes only CG-to-TA transitions. He picked one mutant colony from each mutagenesis plate. He also recovered an spontaneous mutant colony from a control plate that did not contain any mutagen. To identify the plate from which each mutant originated, Frank then used the original mutagens to induce reversions for each mutant. The data obtained is shown in the table below. The + indicates that many revertants were observed, and the - indicates that very few or no revertants were observed Based on the data in this table, identify the plate from which each mutant originated 58U Control Plate of origin Mutant 1 Mutant 2 + Mutant 3- Mutant 4 - 5BU plate Control plate HA plate PRO plate Hint 0Prevcus Giv., Up & View SottonCheck Answer Next Ext about us careers privacy policy terms of use contact ush MacBook Air F5 F6 F7

Explanation / Answer

Mutant 1 - HA plate (As only 5BU can revert CG-TA transitions caused by HA mutagen not HA itself)

Mutant 2 - PRO plate (Frame shift mutations can only be reverted by Proflavin)

Mutant 3 - Control plate (Appears to be a spontaneously induced mutation which none of the mutagens could revert back)

Mutant 4 - 5BU plate ( 5BU can revert both CG-TA and TA-CG transitions, where as HA can revert TA-CG transitions caused by 5BU treatment)

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.