5. (2.5 pts) After growing your cells in optimum conditions, it is time to do a

Question

5. (2.5 pts) After growing your cells in optimum conditions, it is time to do a viability study. You take one flask of cells, collect the contents in 10 ml of new media, remove 1ml to complete your cell count, resulting in the following: GRID 1: 240 Cells GRID 2: 204 Cells GRID 3: 210 Cells GRID 4: 260 Cells (a) Calculate the concentration of cells/ml: (b) Using your stock cell solution, determine the volumes needed to make the following: 1. (2) 6-well plates with 300,000 cells/well. 2. (1) 24-well plate with 100,000 cells/well. 3. (2) 12-well plates with 200,000 cells/well.

Explanation / Answer

Solution--

Cells/ml = the averag count per square * dilution factor * 10^4 [ count 10 squares]

Grid 1-- 240 *5 * 10^4 = 1.2 * 10^7 cells/ml

Grid 2-- 204 * 5 * 10^4 = 1.02 * 10^7 cells/ml

Grid 3-- 210 * 5 * 10^4 = 1.05 * 10^7 cells/ml

Grid 4 -- 260 * 5 * 10^4 = 1.3 * 10^7 cells/ml

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