SIM TUBE explain the properties of this test below. include how this media can h
ID: 202901 • Letter: S
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SIM TUBE explain the properties of this test below. include how this media can help differentiate gram negative organisms. also give a description of how to use it, and interpretation of results.
hoose to grow on this media? rppen ests SIM TUBE: Explain the properties of this test below: Include how this media can help differentiate gram negative organisms. Also give a description of how to use it, and interpretation of results. gram egative organisms: pruaucea no color amonge pon adaition Kovacs Reagent How+o uSe suTUSE TESTExplanation / Answer
SIM Medium is used to differentiate gram-negative bacteria on the grounds of production of sulfide, indole and motility.
The medium contains sodium thiosulfate and ferrous ammonium sulfate which act as indicators for the generation of hydrogen sulfide. Hydrogen sulfide production is recognized when a black precipitate, ferrous sulfide, is produced when ferrous ammonium sulfate reacts with H2 S gas.
Casein peptone, are other components of SIM Medium which are rich in tryptophan. Organisms which have the enzyme tryptophanase degrade tryptophan to indole. Indole is detected after the addition of Kovacs Reagent which is followed by incubation of the inoculated medium. Indole couples with p-dimethylaminobenzaldehyde and produces a red band at the top of the medium. A negative indole test produces no colour change on the addition of Kovacs Reagent.
agar addition to the medium provides a semi-solid structure which makes the detection of bacterial motility easier. Motile organisms produce turbidity throughout the medium. Non-motile organisms grow only along the stab line and leave the surrounding medium clear.
SIM Medium is used to differentiate gram-negative bacteria on the grounds of production of sulfide, indole and motility.
The medium contains sodium thiosulfate and ferrous ammonium sulfate which act as indicators for the generation of hydrogen sulfide. Hydrogen sulfide production is recognized when a black precipitate, ferrous sulfide, is produced when ferrous ammonium sulfate reacts with H2 S gas.
Casein peptone, are other components of SIM Medium which are rich in tryptophan. Organisms which have the enzyme tryptophanase degrade tryptophan to indole. Indole is detected after the addition of Kovacs Reagent which is followed by incubation of the inoculated medium. Indole couples with p-dimethylaminobenzaldehyde and produces a red band at the top of the medium. A negative indole test produces no colour change on the addition of Kovacs Reagent.
agar addition to the medium provides a semi-solid structure which makes the detection of bacterial motility easier. Motile organisms produce turbidity throughout the medium. Non-motile organisms grow only along the stab line and leave the surrounding medium clear.
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