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Question

problem set 2-Problem set 2.pdf × 3 of 8 f Facebook https://uoit bbcswebdav/pid-1047741-dt-content-rid-6334328_1/courses/20180 a Search - + Automatic Zoom Albino 4. (5) While studying tryptophan biosynthesis in a fungus you have identified nutritional mutants that you name trp1 to trp5; these will grow if tryptophan is added to the growth medium. Some of the mutants will also grow if metabolic intermediates are added to the growth medium. The growth characteristics of the mutants are summarized in the table below; + growth,-= no growth. Supplement added to media MinimalChorismate Anthranilate trp1 a. Draw a pathway for tryptophan biosynthesis based on the data above b. What intermediate do you expect to accumulate in each of the mutants? 12:31 AM O Type here to search f ^4x ENG 2018-02-28

Explanation / Answer

Tryptophan biosynthetic pathway:

Chorismate -----> Anthranilate -----> N-(5-phosphoribosyl)-Anthranilate (PRA) -----> 1-(O-Carboxyphenylamino)-1-deoxyribulose-5-phosphate (CDRP) -----> Indole-3-glycerol phosphate (IGP) -----> Indole -----> Tryptophan

1. Anthranilate -----> N-(5-phosphoribosyl)-Anthranilate (PRA)

This reaction is catalyzed by trp5. In trp5 mutant, Anthranilate is accumulated.

2. PRA -----> CDRP

This reaction is catalyzed by trp3. In trp3 mutant, PRA is accumulated.

3. CDRP -----> IGP

This reaction is catalyzed by trp4. In trp4 mutant, CDRP is accumulated.

4. IGP -----> Indole

This reaction is catalyzed by trp2. In trp2 mutant, IGP is accumulated.

5. Indole -----> Trp

This reaction is catalyzed by trp1. In trp1 mutant, Indole is accumulated.

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