b. A sequencing reaction has a ratio of 300 molecules of dATP\'s to 1 molecule o

Question

b. A sequencing reaction has a ratio of 300 molecules of dATP's to 1 molecule of ddATP. What is the probability that termination will occur for a given position in the growing strand when it is opposite a Tin the template strand? In other words, what percentage of the time will a ddATP be incorporated versus a dATP in the growing strand? 8. Double stranded DNA will convert to single-stranded DNA if heated high enough; this temperature is known as the "DNA melting point."Which DNA sequence would you expect to have a higher DNA melting point, one with 6096 GC or one with 45% GC? Both are the same number of base pairs long. Explain your answer based upon the number of hydrogen bonds a GC base pair can form (versus an AT base pair). Diagram the respective stages of the mitotic or meiotic cell cycle of a cell that has a diploid (2N) number of six chromosomes. Use different colored pens or pencils. Indicate some crossing over events. 9. MITOSIS prophase MELOSIS prophase I metaphase I anaphase II metaphase anaphase anaphase

Explanation / Answer

Melting temperature is the temperature required to break the hydrogen bonds between nitrogen bases of DNA to make it single stranded.

Melting temperature is more when a DNA contains more GC pairing. GC rich DNA requires more energy to break the bonds between G and C. since G and C paired by 3 hydrogen bonds more energy is required to separate them. Where as A and T paired by two hydrogen bonds, they require less energy to break the bonds between them.

So the DNA with 60% GC need high melting temperature than 45%GC.

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