understanding of the exercise. In our lab prep space, we have the following solu
ID: 202064 • Letter: U
Question
understanding of the exercise. In our lab prep space, we have the following solutions and powders: sodium citrate (FW = 437 g/mol) SDS powder (FW 288.38 g/mol) 0.5 M EDTA IPTG powder (FW 238.3 g/mol) 20 mg/mL X-gal solution 000X ONPG Premade LE Premade LB agar 0.5 mM Colchicine 50X gel running buffer Agarose powder 95% ethanol 100 mg/mL Ampicillin 10X BamHI restriction enzyme digestion buffer milliQ water dimethylformamide (DMF) Example 1. (DNA isolation lab) How would you make 100mL of 0.151 001 MEDTASolution (one solution that contains all of these reagents)?
Explanation / Answer
Answers provided are to the best of my knowledge.
A15) Take pre prepared LB media + 1000uL X-Gal + 11.9mg of IPTG and make up the volume to 500mL with LB media.
A16) You will add 8mg of XGAL to 400uL of water to make 20mg/ml solution.
A17) With 0.5g of sodium citrate, you can make 57.21 mL solution with concentration 0.2M.
A18) Concentration for 1000x would be 0.013M x 1000 = 13.0M..
A19) You will need to dissolve 5uL of ONPG in 4.995uL water
A20) From a stock of 0.5M, take 50mL and dissolve in 200mL water to make 0.1M 250mL solution.
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