345 30 EFFECTS OF ULTRAVIOLET RADIATION b. Are there any colonies on the unexpos

Question

345 30 EFFECTS OF ULTRAVIOLET RADIATION b. Are there any colonies on the unexposed part of the plate? If so, explain these results e. Does the section that received the most UV exposure have fewer colonies than any other section of this plate? If so, explain these results. 2. Closely examine the MIN + H plate. Is there evidence here of significant killing correlated with the length of UV exposure? What is your evidence for this? After what time period of exposure did the killing become noticeable? seconds 3. What is the effect of UV radiation on mutation frequency?

Explanation / Answer

B) Yes, there are few colonies present on the unexposed part of the plate because bacteria are contagious in nature. It is very easy to spread and since the UV is not given, the existing bacteria also do no die.

c) Yes, the part that received most UV exposure has few colonies as compared to parts that have not received the UV because UV destroys the bacteria depending on the time of the exposure. If it is given for shorter duration, it does not completely destroy the bacteria but longer exposure might cause complete mortality.

Ans 2)

Yes, the evidence is that the place where UV is exposed for longer duration has less colonies as compared to places where UV is exposed for shorter time. For causing complete mortality of the bacteria, a proper and longer exposure is needed.

The noticeable killing is observed after around 60 seconds which tends to alter the mechanism of the bacteria.

Ans 3) UV is also known as mutagen and it highly is in sync with causing mutation of the nucleotides. The frequency of mutation is as high as 50% with presence of UV

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