Woozles have a single pair of chromosomes (n=1) and all sets of alleles are pseu

Question

Woozles have a single pair of chromosomes (n=1) and all sets of alleles are pseudoautomal. Purple (P_) skin color is dominant to red (pp). Yellow (Y_) spots are dominant to green (yy). Blue (B_) tongues are dominant to plaid (bb). In order to map these genes you preform a cross between a woozle that is heterozygous for all three traits, and a woozle that is homozygous recessive for all three traits. The results are:

Purple,yellow,blue: 349

Purple, yellow, plaid: 47

Purple, green, blue: 96

Purple, green , plaid: 1

Red, yellow, blue: 2

Red, yellow, plaid: 101

red, green blue: 50

Red, green, plaid: 354

What are the parental or nonrecombinant offspring?

What is the order of the genes on the chromosome?

Construct a linkage map indicating distances between loci on the map Using your map distances, calculate the expected likelihood of a double cross over Show all work

Using the observed number of double crossovers and your calculated expected number of double crossovers calculate the coefficent of coincidence and the interference

Explanation / Answer

The parental or non recombinant are the one with highest frequency i.e., Purple,yellow,blue: 349 and Red, green, plaid: 354

Order of gene is - Y P B

Distance between two genes = ((Number of Single cross over + number of double cross over)/ total number of progeny)*100

Distance between Y and P = (101 + 96 + 1+ 2)/ 1000 *100 = 20 mapunit

Distance between P and B = (47 + 50 + 1+ 2)/1000*100 = 10 mapunit

Expected DCO = (0.20*0.10*1000) = 20

Interference = 1 - (Observed DCO/ Expected DCO)

Interference = 1-(3/20) = 0.85

Coefficient of coincidence = 1 - interference

Coefficient of coincidence = 1 - 0.85 = 0.15

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