SECTION QUESTIONS Preparing liquid media Completion 1. Microbiologists use the t
ID: 201219 • Letter: S
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SECTION QUESTIONS Preparing liquid media Completion 1. Microbiologists use the term growth to refer to 2. Another term (or descriptive phrase) used for vitamins, amino acids, and other complex organic compou nds required for growth is 3. A microbe that requires many preformed organic compounds for growth is said to be 4. The thing that differentiates complex media from defined media is that the exact chemical composition of a complex medium is 5. Two common components of complex media are 6. 7. If a defined medium contains glucose, ammonium sulfate, potassium phosphates, manganese sulfate, and magnesium and is the name of a sugar that is often added as an energy source to both complex and defined media. sulfate, the cell must be using 8. Another name for a liquid medium is a 9. Sterilization is defined as as its carbon and energy source. True-False (correct all false statements 1. Most common media used by microbiologists today are supplied as premixed powders that need only to be rehydrated and sterilized before use._ 2. Peptone is a general term that refers to enzymatically hydrolyzed protein, regardless of its source. 3. The protein in milk is called trypticase 4. Trypticase peptone specifically refers to enzymatically hydrolyzed casein. 5. Tubes and flasks should be capped or plugged immediately after autoclaving to prevent airborne contaminants from growing in the sterile medium.Explanation / Answer
1. Answer : Growth means the multiplication or increase in population of the microbial cells. The microbial cells continuously divide in number when sufficient nutrients are provided.
2. Answer : Growth factors.
Microorganisms cannot synthesize organic chemicals like amino acids, vitamins and other organic compounds and they are supplied as growth medium.
3. Answer : Chemo-heterotroph.
Proteins, amino acids, carbohydrates, fatty acids and other organic compounds are supplied as growth medium as they cannot synthesize on their own from available inorganic source.
4. Answer : Not known.
Composition of defined media is known but the composition of complex (undefined) media is not known as it differs based on the requirements. The complex media may contain peptone, yeast extract, blood and soy extract.
5. Answer : Peptone and yeast extract
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