Don\'t worry about the drawing in part A. More concerned with B and C 4. Conside

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Don't worry about the drawing in part A. More concerned with B and C

4. Consider the consequences if there were a DNA polymerase that could work by adding nucleotides to the 5'-end rather than the 3'-end of a primer. Assume that nucleotide triphosphates are unchanged (polyphosphate on the 5' C), and assume that the enzyme requires a template and a primer. The elongation step still eliminates pyrophosphate. (A)Describe the coupling process when a new nucleotide is added by this novel polymerase. (Draw a picture analogous to Fig. 8-2 on p. 197 of W+, without the extensive primers nucleophile? What is the leaving group? postulate the existence of an additional new enzymatic activity after proofreading in and templates.) What is the nucleophile? What is the atom being attacked by the (B) If this enzyme had a proofreading function, how would it work? Is it necessary to order to create a proper end for the 5'-elongating polymerase? (C) How would the existence of this enzyme change the organization of the replication fork? (Assume a standard 3'-end elongating polymerase is also around.)

Explanation / Answer

If there is a proofreading function, then after addition of a nucleotide, the enzyme cross checks it. If any wrong nucleotide has been inserted, then it removes those.

Single strand binding protein gets bound and holds the fork.

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