Fluorescent labels present on the 5\' end of the linkers or incorporated in modi

Question

Fluorescent labels present on the 5' end of the linkers or incorporated in modified nucleotides. The fluorescent sample is then incubated with a DNA microarray in order to determine which sequences were isolated in the procedure. This microarray or chip gives rise to the second "chip" in the name G. Is this experiment an example of a blot or a reverse blot? Explain how one learns which sequences the DNA binding protein recognizes. H. Which set of sequences is most appropriate for this experiment. Justify your choice. Every possible sequence for the length probe used (typically 25 nt) Regularly spaced sequences representing all nonrepetitive sequences from the chromosome of the organism being studied .All transcribed sequences made by making cDNA from total cellular RNA from any practical group of cells from the organism .All transcribed sequences made by making cDNA from total cellular RNA from the tissue under study All sequences in mature mRNAs, made from polyA tailed mRNAs isolated from all cells form the organism being studied All sequences in mature mRNAs, made from polyA tailed mRNAs isolated from the tissue under study

Explanation / Answer

G. This experiment is an example of blot because its sequencing from 5' end to 3' end.

H. Answer:- all transcribed sequence made by making cDNA from total cellular RNA from any practical group of cells from the organism.

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