A complementation experiment was performed with S. cerevisiae cells possessing a

Question

A complementation experiment was performed with S. cerevisiae cells possessing a temperature-sensitive cdc28 mutation. Wild-type cells, cdc28 cells, and cdc28 cells transformed with the maize cdc2 cDNA under the influence of a strong promoter were grown at the permissive (25 °C) or restrictive (37 °C) temperatures. Cell proliferation was monitored by the growth of colonies on the culture plates (shown in the figure on the next page) 25 °C 37 °C Wild type cdc 28tsWild type cdc 28ts cdc 28ts +maize cdc2 cdc 28ts+maize cdc2 Why do the cdc28- cells form colonies at 25 °C but not 37 °C? What is the significance of colony formation of the cdc28- + maize cdc2 cells at 37 °C? What does this experiment tell us about the functional homology of cyclin-dependent kinase genes among eukaryotic species?

Explanation / Answer

cdc28 is a temperature sensitive mutant, and the gene cdc28 is required for survival at 37 °C (No growth in mutant) at the same time we observed growth at 25 °C permissive temperature, where gene may be nonfunctional or not required for the growth.     At 37 °C we are able to see the cdc28 cells which were transformed with maize cdc2 were grown in comparison to cdc28 where no growth were observed suggesting that maize cdc2 can complement the function of cdc28 and help in the survival of the cells. Maize cdc2 is an eukaryotic gene which can be functionally same as with prokaryotes.

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