2. As genes encoding cyclin-dependent kinases were cloned from fission yeast, bu

Question

2. As genes encoding cyclin-dependent kinases were cloned from fission yeast, budding yeast, and animal cells, investigators working in plant systems designed experiments to determine whether plants also possessed CDKs that functioned as the catalysts for cell predicted amino acid sequences of human cdc2, S. pombe cdc2, and S. cerevisiae cdc28 synthesized by PCR using these primers and maize cDNA library as a template Why were the boxed regions selected as the basis for primer design? S. pombe cdc2 human cdc2 S. cerevisiae cdc28 MENYOKVEKIGEGTYGVVYKARHKLSG---RIVAMKKIRLEDESEGVPSTAIREIS QVVAMKKIRLESEEEGVPSTAIREIS MSGELANYKRLEKVGEGTYGVVYKALDLRPGOGORVVALKKIRLESEDEGVPSTAIREIS MEDYTKIEKIGEGTYGVVYKGRHKTTG S. pombe cdc2 LLKEVNDENNRSNCVRLLDILHAES-KLYLVFEFLDMDLKKYNDRISETGATSLDPRLVQ HPNIVSLODVLMODS-RLYLIFEFLSMDLKKYLDSIPPG-QYMDSSLVK S, cerevisiae cdc28 LLKELK DDNlVRLYD1VHSDAHKLYLVFEFLDLDLKRYMEGIPKD--QPLGADIVK uman cdc2 LLKELR S. pombe cdc2 uman cdc2 SYLYOILOGIVECHSRRVLHRDLKPONLLIDDKGTIKLADFGLARAFGIPIRVYTHEVV S. cerevisiae cdc28 KFMMOLCKGIAYCHSHRILHRDLKPONLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVT S. cdc2 LWYRAPEVLLGSRHYSTGVDIWSVGCIFAEMIRRSPLFPGDSEIDEIFKIFOVLGTPNEE uman cdc2 LWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNE cdc2 uman cdc2 VWPGVTLLODYKSTFPRWKRMDLHKVVPNGEEDAIELLSAMLVYDPAHRISAKRALOQNY VWPEVESLODYKNTFPKWKPGSLASHVKNLDENGLDLLSKNLIYD PAKRISGKMALNHPY IWPDIVY L PDFKPSFPOWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHP Y S. cerevisiae cdc28 S. pombe cdc LRDPH uman cdc2 FNDLDNQIKKM S, cerevisiae cdc28 FQES

Explanation / Answer

1. The boxed regions were chosen as those regions were conserved in the Cyclin dependant kinases in human, pombe and cerevisiae .If you notice closely those particular regions are the same in all three species and hence a hypothesis can be made if cdk were to be present in plants this particular region would be the same.

2. the level of similarity is greater than 60 % for both this suggest that only certain parts of the genes have changed evolutionary but the functional part has remained the same in mosty all three and 60 % similarity is high enough for to prove the gene has been conserved

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