55 tor Canvas Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILIN
ID: 200708 • Letter: 5
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55 tor Canvas Word Nth Raba HILE HOME INSER DESIGN PAGE LAYOUT REHERENYES MAILINGS REVIEWVIEW Cut Anal a Copy Paste , u_x x A,2- , ,n, Ou te- 1 Nofmal 1 No Spac. Heading 1 Heading 2 Tile Subtitle subtle Ern. Emphasis Intense E. Strong r Format Pinte Select. sly es Question 3. (6 pts) In cass, we described 3 differen types of DNA arkers. a) Please list them, give full names and a description of each one Identfy the most commonly used or appropriate technology to analyze and detect polymorphism in each one of the three DNA markers mentioned above b) Question a (4 pts) How has the Rt protein been used to increase insect-resistance in plants? Include in your answer its function, its arigin, the "meaning" of and why the Rt protein is not toxic to humans. 1151 AM O Type here to searchExplanation / Answer
Question 3:
The answer is
a. RFLP - Restriction Fragment Length Polymorphism
To study the variations in the DNA length generated by endonuclease treatment.
b. AFLP - Amplified Fragment Length Polymorphism
To study the variation in the restriction fragment lengths which is caused by Single Nucleotide Polymorphisms or insertion or deletion of basess which create or abolish restriction site of endonucleases.
c. RAPD - Random Amplified Polymorphic DNA
To study the variations in organisms in randomly manner though Polymease chain reaction.
b)
The most commonly used technology
a. RFLP - Restriction endonuclease diagestion and gel or capillary electrophoresis.
b. AFLP - Restriction endonuclease diagestion, Polymease chain reaction and gel or capillary electrophoresis.
c. RAPD - Polymease chain reaction and gel or capillary electrophoresis.
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