(7) Which of the enzymes from the chart below produce identical sticky ends? Res

Question

(7) Which of the enzymes from the chart below produce identical sticky ends? Restriction Recognition GATCC Acatc endonuclease sequence Alul AG/CT TCGA G BamHI G/GATCC GATCT BglII A/GATCT AT Clal AT/CGAT EcoRI G/AATTC | HindIII A/AGCTT AAT Pstl CTGCA/G Sacl GAGCT/C Xbal T/CTAGA ETAGA C GAT AG and the (8) The graph on the left shows the titration curve of graph on the right shows the titration curve of 12 - 10 10- pH pH A " " 0.5 1.0 1.5 2.0 2.5 3.0 0.5 1.0 1.5 2.0 2.5 3.0 moles OH-/moles amino acid moles OH-/moles amino acid A) arginine; glutamine (B) glycine; aspartate C) histidine; arginine D) serine; histidine

Explanation / Answer

7.AluI, BamHI, BglII, EcoRI, HindIII, PstI, XbaI produces sticky ends.

Explaination: Endonuclease are enzymes that produces internal cuts, called cleavage, in DNA molecule. It cleaves DNA only within or near those sites, which have specific base sequences of 4-8 nucleotides and cleave such endonuclease are known as restriction endonuclease & site recognised by them are called recognition sequences or recognition sites.The cut DNA fragments by restriction endonuclease may have mostly sticky ends or blunt ends.

There are 4 types of restriction nuclease i.e. type I, type II, type II, Type IIs. Each restriction sequence has two fold rotational symmetry i.e. the same nucleotide sequence occurs on both strands of DNA which run in opposite direction which refered to as palindromes. The single-stranded sticky DNA end can easily pair with any other DNA fragment having complementary sitcky ends.

Restriction endonuclease with their sources & sequence.

AG/CT

TC/GA

G/G AT CC

CTTA AA/G

A/G ATCT

TCTAG/A

G/ AA TTC

C TT AA/G

A/A GCTT

TTCG A/A

CTGCA/G

G/A CGTC

8. B. glycine ; aspatate

Restriction Endonuclease Source (Organisn and Strain) Recognition sequence AluI Arthrobacter luteus

AG/CT

TC/GA

BamHI Bacillus aymloliquefaciens H

G/G AT CC

CTTA AA/G

BaglII Bacillus globigli

A/G ATCT

TCTAG/A

EcoRI Escherichia coli Ry 13

G/ AA TTC

C TT AA/G

HindIII Haemophilus influenza RD

A/A GCTT

TTCG A/A

PstI Providencia stuartii

CTGCA/G

G/A CGTC

XbaI Xanthomonas badrii T/CTAGA

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