Shotgun sequencing is a method of DNA sequencing in which: a. probes are used to
ID: 200222 • Letter: S
Question
Shotgun sequencing is a method of DNA sequencing in which:
a. probes are used to find genes of interest
b. computers are used to find overlapping sequences
Which technology makes it possible to monitor the expression of thousands of genes simultaneously?
a. Pyrosequencing
b. Proteomics
c. DNA microarrays
d. Illumina analysis
Which process is used to separate mixtures of protiens?
a. ChIP
b. Two-Dimentional Gel Electrophoresis
c. Posttranslational Covalent Modification
d. Isoelectric Field Migration
Which technique identifies the amino acid composition of small peptides?
a. Pyrosequencing
b. Mass Spectrometry
c. The Sanger Method
d. Two-Dimensional Gel Electrophoresis
Which type of analysis can be used to examine several possible reading frames in the DNA sequence?
a. Pyrosequencing
b. DNA Microarray
c.Three-Dimensional Gel Electrophoresis
d.ORF Analysis
Homologous sequences have a common ancestor and will carry the same types of sequences. When homologous sequences are found in different species, they are orthologs which evolve through divergence. When two homologous sequences are found in a single organisms they are paralogs which arise by duplication. Is a gene family orthologous or paralogous?
a. Orthologous
b. Paralogous
c.Could be either one
d. Is neither
Explanation / Answer
Shotgun sequencing is a method of DNA sequencing in which computers are used to find overlapping sequences.
Scientists using DNA microarrays for measuring the expression levels of large numbers of genes simultaneously.
Two-Dimentional Gel Electrophoresis is used to separate mixtures of protiens. In short it is known as 2-DE or 2-D electrophoresis.In this process Proteins are analysed and get separated by two properties in two dimensions on 2D gels.
ORF (Open Reading Frame) Analysis is used to examine several reading frames in the DNA Sequence.In ORF analysis folloiwng tools are used:
1. ORF Finder
2. ORF Investigator
3. ORF Predictor
Gene families could be either paralogs or orthologs. It is related genes that share a common ancestor.Paralogs are genes with similar sequences from within the same species while orthologs are genes with similar sequences in different species.
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