Despite the potential of any other techniques, I have been forced to use a combi

Question

Despite the potential of any other techniques, I have been forced to use a combination of size exclusion chromatograghy and ion exche Which of the following statements must have been true? Pick ALL that apply A Iwould use a water based mobile phase of 7 4 B. I would use an acetonitrile mobile phase with a pH of 7 4 C I would use a water based mobile phase of pH 72 D-I would use an acetonitrile phase of 72 E-| would salt it out F would obtain my soluble protein fraction in native conformation by dialysis to remove salts or to GI would obtain my soluble protein traction by eluting from the column in stages slowiny changing the pH

Explanation / Answer

The answers are d, g. On using the acetonitrile as the mobile phase at pH of 7.2 it tends to carry along the solute molecules through the day. Also by slowly changing the pH it becomes easy and convenient for the solute to pass along the mobile phase.

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