I\'m pretty sure I selected the right answer, but I\'m unsure QUESTION 2 My boss

Question

I'm pretty sure I selected the right answer, but I'm unsure

QUESTION 2 My boss has given me the purified extracellular portion of the Notch receptor. I have been told I need to purify Delta to keep my job. I have a problem. Delta and Notch bind but do not catalyze a chemical reaction with easy to identify products. The only way to know if they work is to know if they bind together. I am smart! So… not a problem! Following purification, I would likely do which of the following to assay if Delta binds to Notch? Pick ALL that apply. I would look to see if the individual proteins have changed size on a denaturing gel. I would see if ADP were produced using an assay for luciferase activity (light from the firefly protein). I would use FRET and label Delta with the donor chromophore (light producing) and Notch with the acceptor molecule (light quenching). I would look for the generation of more light. I would look for the quenching of light (less light).

Explanation / Answer

The correct option are options (a), (b) and (c).. These are the methods you can use for finding the interactions between the two i.e delta and notch.

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