6. Which of the following statements is NOT true about agarose gel electrophores
ID: 199708 • Letter: 6
Question
6. Which of the following statements is NOT true about agarose gel electrophoresis? a) It results in the separation of DNA fragments based on size b) It relies on the migration of negatively charged DNA molecules toward the positive electrode c) Larger DNA fragments tend to migrate further along in the gel than the smaller fragments because t pieces of DNA carry more negative charge d) The porosity (pore size) of the agarose gel depends on the concentration (percentage by volume) of agarose used he bigger 7. The sequence recognized by the restriction enzyme to cut the DNA is called a) recognition site b) restriction site c) ori site d) both A and B are correct TTCG 8. What characteristic do all three restriction sites have in common? a) they are all six-nucleotide sequences b) they are all palindromic sequences c) the restriction sites are GC-rich sequences d) upon cleavage, they all generate overhangs 9. Which of the restriction enzymes show symmetric cleavae? a) Haelll b) EcoRI c) Hindlll D) both EcoRI and HindIII 10. Which of the restriction enzymes generate 5' overhangs? a) Haelll b) EcoRI c) Hind III d) Both EcoRI and HindIIIExplanation / Answer
Answers are provided below:
6) C
7)B
8)B
9)A
10)D
11)D
12)A
13)D
14)A
15)B
16)A
17)B
18)D
19)C
20)B
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