Which statements apply to which coat proteins (check all that apply for each coa

Question

Which statements apply to which coat proteins (check all that apply for each coat)? Statement COPII COPI clathri Protein coat dissociates after vesicle is formed Functions in retrograde transport from Golgi to ER After coat dissociation, fusion is facilitated by Rab and SNARE proteins Has a role in transport of lysosomal enzymes, like acid hydrolases Is essential for exocytosis Assembly regulated by the ARF family of small G-proteins Unpolymerized form has a triskelion structure Found on vesicles that have KDEL receptors unbound to KDEL ligand Translation of proteins across rough microsomal membranes can be judged by several criteria including (1) cleavage of signal sequences by signal peptidase, (2) glycosylation of parts of the protein in the lumen by enzymes in the microsome (ER), and (3) protection from protcases of any part that is transmembrane or fully in the lumen and protection from de-glycosylation enzymes like endolH unless detergents are present to solubilize the lipid bilayer In an in vitro translation system to which you can add microsomes, you translate a protein and then subject it to the ndicated treatments. Each reaction was then analyzod by SDS-PAGE and the results are shown below A. Draw the protein in the context of the microsome. Indicate the microsomal membrane and lumen and the N- and C-lermini ABSENT PRESENT protease detergent endo H B. From amino to carboxyl terminus, what topogenic sequences would lead to the arrangement of this protein.

Explanation / Answer

Protein coat dissociate after vesicle formation-clathrin

COP I

All three

COPII

Clathrin

Clathrin andCOPI

Clathrin

COPI

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