Exercise 13 1. What is a chromophore? 2. Why is negative staining considered an

Question

Exercise 13 1. What is a chromophore? 2. Why is negative staining considered an indirect staining of the cell? 3. Negative stains are acidic stains. Define acidic stain 4. Give an example of two dyes used for negative staining 5. List the four steps for a negative staining technique using an inoculating needle Exercise 16 1. What two genera of bacteria form endospores when they exhaust essential nutrients? 2. Give 3 differences between a vegetative cell and an endospore (based on lab manual p119) 3. What dyes can be used to stain the endospore? 4. What method is used to destroy endospores? 5. What is the difference between the Schaeffer method and the Dorner method of staining endospores Exercise 13 1. What is a chromophore? 2. Why is negative staining considered an indirect staining of the cell? 3. Negative stains are acidic stains. Define acidic stain 4. Give an example of two dyes used for negative staining 5. List the four steps for a negative staining technique using an inoculating needle Exercise 16 1. What two genera of bacteria form endospores when they exhaust essential nutrients? 2. Give 3 differences between a vegetative cell and an endospore (based on lab manual p119) 3. What dyes can be used to stain the endospore? 4. What method is used to destroy endospores? 5. What is the difference between the Schaeffer method and the Dorner method of staining endospores Exercise 13 1. What is a chromophore? 2. Why is negative staining considered an indirect staining of the cell? 3. Negative stains are acidic stains. Define acidic stain 4. Give an example of two dyes used for negative staining 5. List the four steps for a negative staining technique using an inoculating needle Exercise 16 1. What two genera of bacteria form endospores when they exhaust essential nutrients? 2. Give 3 differences between a vegetative cell and an endospore (based on lab manual p119) 3. What dyes can be used to stain the endospore? 4. What method is used to destroy endospores? 5. What is the difference between the Schaeffer method and the Dorner method of staining endospores

Explanation / Answer

Answer for question number 1 (Excercise 13)

Chromophore is any part of the molecule that is responsible for the colour of that molecule or a compound. Chromo means colour and the phoros means bearer. Examples of chromophores are carotenoids,

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