Specs Support ptop. Write on. 080p easy ar Name: Bio 382-1 Homework Assignment #

Question

Specs Support ptop. Write on. 080p easy ar Name: Bio 382-1 Homework Assignment #3 You are testing the basic requirements for base excision repair by setting up a complete reaction in a test tube using purified proteins and a plasmid containing a damaged guanine nucleotide (oxo-G). The damaged oxo-G base is present in the following sequence in red: 5'-GAATTc-3. 3 ,-CTTAAG-5 , This sequence is present at position (*) in the plasmid pictured below: 3.5 kb 9.0 kb BamHI 1) Which restriction enzyme could be used to determine whether this damaged base is successfully repaired, and cuts at position (') in the plasmid? (1 point) EcoR 2) Which sized band(s) would you expect to see on an agarose gel after: (0.5 points each)

Explanation / Answer

1 answer. EcoR1 because EcoR1 is a the restriction enzyme that recognize 5'-GAATTC-3'. When base excision repair corrected the Oxo G then EcoR1 will cut the plasmid and will give two band. But when the repair is not sucessfull EcoR1 will not cut the plasmid.

2 in agarose gel two band of 3.5 kb and 5.5kb will be visible when cut with both EcoR1 and BamH1 and a single band of 9 kb when cut with EcoR1.

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